rabbit polyclonal anti glun2c antibodies  (Novus Biologicals)

Journal: Journal of Cardiovascular Pharmacology

Article Title: Cardiac N-methyl d-aspartate Receptors as a Pharmacological Target

doi: 10.1097/fjc.0000000000000424

Figure Lengend Snippet: FIGURE 7. Confirmation of the presence of GluN1, GluN2A, GluN2B, and GluN2C proteins in rat heart. Rat brain tissue homogenate was used as a control. Panel A shows the repre- sentative images in which the subunits were detected by immunoblotting. B, Changes in the GluN2B protein abun- dance in the heart with age of the animal. Actin was used as a loading control.

Article Snippet: Staining was performed with the following primary antibodies: mouse monolconal anti-GluN1 antibody, and rabbit polyclonal anti-GluN2C antibodies (NB300-118 and NB300-107; Novus Biologicals), and rabbit polyclonal anti-GluN2A and mouse monoclonal anti-GluN2B antibodies (ab14596 and ab28373; Abcam).

Techniques: Control, Western Blot

Journal: Translational Psychiatry

Article Title: Environmental enrichment prevents the late effect of acute stress-induced fear extinction deficit: the role of hippocampal AMPA-GluA1 phosphorylation

doi: 10.1038/s41398-020-01140-6

Figure Lengend Snippet: Stressed SC-housed animals showed higher expression of pGluA1 ( A ) and GluA1 ( B ) in the HC comparing to SC-housed, unstressed (only in B ), EE-housed, stressed and unstressed animals. EE-housed animals (both stressed and unstressed) presented the same expression levels of pGluA1 ( A ) and GluA1 ( B ) than SC-housed, unstressed animals. There were no differences in the GluN2B ( C ) and GluN2C ( D ) expression in both SC- and EE-housed animals (stressed or unstressed). There were no differences in the synaptophysin ( E ) and PSD-95 ( F ) expression in both SC- and EE-housed animals, regardless of the presence of stress. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A ; n = 6 for each group in B , D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test. Significance differences between groups are indicated as * p < 0.05 and ** p < 0.01.

Article Snippet: Blots were blocked with 5% bovine serum albumin (BSA) diluted in TBS-T buffer (50 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.5) for 1 h at room temperature, and subsequently incubated overnight at 4 °C with specific primary antibodies: polyclonal rabbit anti-GluA1 (AB1504, dilution 1:2000; EMD Millipore Corporation), polyclonal rabbit anti-Ser845phospho-GluA1 (AB5849, dilution 1:1000; EMD Millipore Corporation), polyclonal rabbit anti-GluN2B (#454582, dilution 1:1000; EMD Millipore Corporation), polyclonal rabbit anti-GluN2C (#454584, dilution 1:1000; EMD Millipore Corporation), monoclonal mouse anti-synaptophysin (AB8049, dilution of 1:20,000; Sigma-Aldrich Corporation), and monoclonal mouse anti-PSD-95 (AB99099, dilution of 1:20,000; Abcam).

Techniques: Expressing, Autoradiography, Western Blot

Journal: Translational Psychiatry

Article Title: Environmental enrichment prevents the late effect of acute stress-induced fear extinction deficit: the role of hippocampal AMPA-GluA1 phosphorylation

doi: 10.1038/s41398-020-01140-6

Figure Lengend Snippet: There was an environmental effect on the pGluA1 ( A ) expression in the BLA, but there were no effects of either environment or stress on the expression of GluA1( B ), GluN2B ( C ), GluN2C ( D ), synaptophysin ( E ), and PSD-95 ( F ) in the same brain area. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A and B ; n = 6 for each group in D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test (no differences were detected between the experimental groups). ANOVA significant main effect is indicated as ## p < 0.01.

Article Snippet: Blots were blocked with 5% bovine serum albumin (BSA) diluted in TBS-T buffer (50 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.5) for 1 h at room temperature, and subsequently incubated overnight at 4 °C with specific primary antibodies: polyclonal rabbit anti-GluA1 (AB1504, dilution 1:2000; EMD Millipore Corporation), polyclonal rabbit anti-Ser845phospho-GluA1 (AB5849, dilution 1:1000; EMD Millipore Corporation), polyclonal rabbit anti-GluN2B (#454582, dilution 1:1000; EMD Millipore Corporation), polyclonal rabbit anti-GluN2C (#454584, dilution 1:1000; EMD Millipore Corporation), monoclonal mouse anti-synaptophysin (AB8049, dilution of 1:20,000; Sigma-Aldrich Corporation), and monoclonal mouse anti-PSD-95 (AB99099, dilution of 1:20,000; Abcam).

Techniques: Expressing, Autoradiography, Western Blot

Journal: Translational Psychiatry

Article Title: Environmental enrichment prevents the late effect of acute stress-induced fear extinction deficit: the role of hippocampal AMPA-GluA1 phosphorylation

doi: 10.1038/s41398-020-01140-6

Figure Lengend Snippet: There was an environmental effect on the pGluA1 ( A ) expression in the FC, but there were no effects of either environment or stress on the expression of GluA1 ( B ), GluN2B ( C ), GluN2C ( D ), synaptophysin ( E ), and PSD-95 ( F ) in the same brain area. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A and B ; n = 6 for each group in D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test (no differences were detected between the experimental groups). ANOVA significant main effect is indicated as # p < 0.05.

Article Snippet: Blots were blocked with 5% bovine serum albumin (BSA) diluted in TBS-T buffer (50 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.5) for 1 h at room temperature, and subsequently incubated overnight at 4 °C with specific primary antibodies: polyclonal rabbit anti-GluA1 (AB1504, dilution 1:2000; EMD Millipore Corporation), polyclonal rabbit anti-Ser845phospho-GluA1 (AB5849, dilution 1:1000; EMD Millipore Corporation), polyclonal rabbit anti-GluN2B (#454582, dilution 1:1000; EMD Millipore Corporation), polyclonal rabbit anti-GluN2C (#454584, dilution 1:1000; EMD Millipore Corporation), monoclonal mouse anti-synaptophysin (AB8049, dilution of 1:20,000; Sigma-Aldrich Corporation), and monoclonal mouse anti-PSD-95 (AB99099, dilution of 1:20,000; Abcam).

Techniques: Expressing, Autoradiography, Western Blot